You need to make sure that the license file is embedded into the resource.  Often Visual Studio will include the correct information in licenses.licx if you use a visual component (such as the DotTwain component) but if you do it all in code, you will need to create this licenses.licx file yourself.  The joys of .NET licensing, most component vendors use this scheme.  Once the license file is embedded into the resource you can deploy to any client. 

Add this line to the licenses.licx file:
Atalasoft.Twain.Acquisition, Atalasoft.DotTwain

Here are a couple KB's on the topic:

http://www.atalasoft.com/kb/Article.aspx?id=10103
http://www.atalasoft.com/kb/Article.aspx?id=10058

Here is documentation on the topic:
http://www.atalasoft.com/products/dotimage/docs/CompilingDotImage.html